I agree with Biffo that you would be unlikely to confuse "No." While using the word "No" at the time you think about it. But I do often working experience a split-2nd hesitation when reading a sentence with "No.", specially if there's a space amongst it and the subsequent amount: https://90s-girl-group-no-scrubs35789.kylieblog.com/37091023/no-scrubs-tlc-an-overview