Objectives : This experimental study was performed to investigate the safety and efficacy of Bovis Calculus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. To identify the use of it as eye drop. the eye irritation test of rabbits and the antibacterial test of Staphylococcus aureus. Staphylococcus epidermidis. https://www.hindirochakkahaniya.com/