The reverse primer is made so the 5´finishes of the two primers anneal backto-again. B) Deletions are engineered by planning normal, non-mutagenic ahead and reverse primers that flank the region being deleted. C) Insertions fewer than or equivalent to 6 nucleotides are integrated in the five´ conclude with the forward https://site-directed-mutagenesis54310.webdesign96.com/22502343/the-definitive-guide-to-site-directed-mutagenesis